The readily-releasable pool dynamically regulates multivesicular release
نویسندگان
چکیده
منابع مشابه
Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse
Recycling of synaptic vesicles (SVs) is a fundamental step in the process of neurotransmission. Endocytosed SV can travel directly into the recycling pool or recycle through endosomes but little is known about the molecular actors regulating the switch between these SV recycling routes. ADP ribosylation factor 6 (Arf6) is a small GTPase known to participate in constitutive trafficking between p...
متن کاملRapid Reuse of Readily Releasable Pool Vesicles at Hippocampal Synapses
Functional presynaptic vesicles have been subdivided into readily releasable (RRP) and reserve (RP) pools. We studied recycling properties of RRP vesicles through differential retention of FM1-43 and FM2-10 and by varying the time window for FM dye uptake. Both approaches indicated that vesicles residing in the RRP underwent rapid endocytosis (tau approximately 1s), whereas newly recruited RP v...
متن کاملReadily releasable pool size changes associated with long term depression.
We have estimated, for hippocampal neurons in culture, the size of the autaptic readily releasable pool before and after stimulation of the sort that produces culture long term depression (LTD). This stimulation protocol causes a decrease in the pool size that is proportional to the depression of synaptic currents. To determine if depression in this system is synapse specific rather than genera...
متن کاملQuantitative analysis of synaptic vesicle release and readily releasable pool size in hippocampal neurons.
In central nervous system only a limited number of vesicles exist in the presynaptic terminals. The size and fusion modes of the vesicles were particularly important because of their potential impact on neuronal communications. Efficient methods were needed to analyze the recycling kinetics of synaptic vesicle and the size of readily releasable pool (RRP). In this study, fluorescent dyes with d...
متن کاملErratum: A Three-Pool Model Dissecting Readily Releasable Pool Replenishment at the Calyx of Held
Although vesicle replenishment is critical in maintaining exo-endocytosis recycling, the underlying mechanisms are not well understood. Previous studies have shown that both rapid and slow endocytosis recycle into a very large recycling pool instead of within the readily releasable pool (RRP), and the time course of RRP replenishment is slowed down by more intense stimulation. This finding cont...
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ژورنال
عنوان ژورنال: eLife
سال: 2019
ISSN: 2050-084X
DOI: 10.7554/elife.47434